The past decade has seen tremendous advances in the study of G protein coupled receptors (GPCRs), including the molecular cloning and identification of more than 100 hundred GPCR genes.
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Cloning of G Protein Coupled Receptor Genes: The Use of Homology Screening and the Polymerase Chain Reaction (A. Marchese, et al.).
Practical Approaches for Cloning G Protein Coupled Receptors Using Mammalian Cell Expression (T. Murphy).
G Protein Coupled Receptor Informatics and the Orphan Problem (K. Lynch).
The Use of Xenopus laevis Oocytes for the Study of G Protein Coupled Receptors (A. Lee & M. Durieux).
Stable Expression of G Protein Coupled Receptors in Mammalian Cells (K. Neve & R. Neve).
Heterologous Expression in Primary Cell Cultures (R. Leonard).
Expression of G Protein Coupled Receptors in Escherichia coli (R. Grisshammer).
Large Scale Expression/Purification of G Protein Coupled Receptors Mammalian Cells (A. Robeva & J. Linden).
High Level Expression of G Protein Coupled Receptors in the Baculovirus/SF9 Cell Expression System (N. Rondeau, et al.).
Expression of G Protein Coupled Receptors in Drosophila Schneider 2 Cells (M. Graziano, et al.).
Heterologous G Protein Coupled Receptors Expressed in Saccharomyces cerevisiae: Methods for Genetic Analysis and Ligand Identification (M. Pausch, et al.).
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