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Nederlands Buitenlands   Alles  Titel  Auteur  ISBN        
Levende natuur
Biologie algemeen

Marilyn S. Arsham

The AGT Cytogenetics Laboratory Manual

€ 236.20

Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics.

Taal / Language : English


Contributing Authors



1. The Cell and Cell Division
Margaret J. Barch and Helen J. Lawce

1.1 The Cell

1.2 The Cell Cycle

1.3 Recombinant DNA Techniques

1.4 The Human Genome


2. Cytogenetics: An Overview
Helen J. Lawce and Michael G. Brown

2.1 Introduction

2.2 History of Human Cytogenetics

2.3 Cytogenetics Methods

2.4 Slide-Making

2.5 Chromosome Staining

2.6 Chromosome Microscopy/Analysis


Contributed Protocols

3. Peripheral Blood Cytogenetic Methods
Helen J. Lawce and Michael G. Brown

3.1 Using Peripheral Blood for Cytogenetic Analysis

3.2 Special Uses of Peripheral Blood Culture

3.3 Peripheral Blood Constituents

3.4 Special Handling

3.5 Cell Culture Equipment and Supplies

3.6 Harvesting Peripheral Blood Cultures

3.7 Chromosome Analysis of Peripheral Blood

3.8 Storage of Fixed Specimens



Contributed Protocols

4. General Cell Culture Principles and Fibroblast Culture
Debra F. Saxe, Kristin M. May and Jean H. Priest

4.1 Definitions of Cell Culture

4.2 Basic Considerations in Cell Culture

4.3 Fibroblast Culture



Additional Readings

Contributed Protocols

5. Prenatal Chromosome Diagnosis
Kristin M. May, Debra F. Saxe and Jean H. Priest

5.1 Introduction

5.2 Amniotic Fluid

5.3 Culture of Amniotic Fluid

5.4 Analysis of Amniotic Fluid

5.5 Chorionic Villus Sampling (CVS)

5.6 Analysis of Chorionic Villus


Contributed Protocols

6. Chromosome Stains
Helen J. Lawce

6.1 Introduction

6.2 Chromosome Banding Methods

6.3 5 -bromodeoxyuridine (BrdU) Methodologies

6.4 T-Banding/CT-Banding

6.5 Antibody Banding and Restriction Endonuclease Banding

6.6 Destaining Slides

6.7 FISH DAPI Bands

6.8 Sequential Staining

6.9 Acknowledgements


Contributed Protocols

7. Human Chromosomes: Identification and Variations
Helen J. Lawce and Luke Boyd

7.1 Understanding the Basics

7.2 Description of Human Chromosome Shapes

7.3 Determination of G-Banded Chromosome Resolution



8. ISCN: The Universal Language of Cytogenetics
Marilyn Schaeffer Arsham and Lisa G. Shaffer

8.1 The Language

8.2 Karyotype

8.3 Numerical Events

8.4 Structural Events

8.5 Derivative Chromosomes (der)

8.5.4 Multiple Events Derivative

8.6 Symbols of Uncertainty

8.7 Random Versus Reportable

8.8 Multiple Cell Lines

8.9 Fluorescence In Situ Hybridization (FISH)

8.10 Microarray (arr) and Region Specific Assay (rsa)



9. Constitutional Chromosome Abnormalities
Kathleen Kaiser-Rogers

9.1 Numerical Abnormalities

9.2 Structural Rearrangements


10. Genomic Imprinting
R. Ellen Magenis

10.1 Introduction

10.2 Human Genomic Disease and Imprinting

10.3 Germ Cell Tumors - UPD and Imprinting


Glossary of Terms

11. Cytogenetic Analysis of Hematologic Malignant Disease
Nyla Heerema

11.1 Introduction

11.2 Leukemia

11.3 B- and T-Cell Lymphoblastic Neoplasms

11.4 Lymphomas

11.5 Laboratory Practices




Contributed Protocols

12. Cytogenetic Methods and Findings in Human Solid Tumors
Marilu Nelson

12.1 Introduction

12.2 Processing Tumor Specimens

12.3 Recurrent Cytogenetic Abnormalities

12.4 Molecular Genetics and Cytogenetics Techniques



Contributed Protocols

13. Chromosome Instability Syndromes
Yassmine Akkari

13.1 Introduction

13.2 Fanconi Anemia

13.3 Bloom Syndrome

13.4 Ataxia Telangiectasia

13.5 Nijmegen Breakage Syndrome

13.6 Immunodeficiency, Centromeric Instability, and Facial Anomalies (ICF) Syndrome

13.7 Roberts Syndrome]

13.8 Werner Syndrome

13.9 Rothmund-Thomson Syndrome]

13.10 Proficiency Testing]



Contributed Protocols

14. Microscopy and Imaging
Margaret J. Barch and Helen J. Lawce

14.1 The Standard Microscope

14.2 Brightfield Microscopy

14.3 Fluorescence Microscopy

14.4 Specialized Microscopy

14.5 Capturing the Microscopic Image


15. Computer Imaging
Christine E. Haessig

15.1 Introduction

15.2 Techniques to Improve Karyogram Image Quality

15.3 Metaphase Preparation

15.4 Microscopy

15.5 Image Capture

15.6 Enhancement

15.7 Advanced Contrast

15.8 Macro Programming

15.9 FISH Imaging

15.10 Printing

15.11 Quality Control

15.12 Archiving


16. Fluorescence in situ Hybridization (FISH)
Helen J. Lawce and Jeff Sanford

16.1 Introduction

16.2 Clinical Applications of FISH Probes

16.3 Deletion/Duplication Probes for Constitutional Abnormalities

16.4 Hematology/Oncology and Solid Tumor Probes

16.5 Sources and Characteristics of Probes Available to the Clinical Cytogenetics Laboratory

16.6 Special Uses of Probes

16.7 Important FISH Probe Adjuvants

16.8 Principles of FISH

16.9 FISH Methods An Overview

16.10 FISH Analysis and Reporting

16.11 FISH Probe Testing and Validation

16.12 FISH for Special Investigation

16.13 Preimplantation Genetic FISH

16.14 Other Applications

16.15 Variants in FISH Signal Patterns




Contributed Protocols

17. Multicolor FISH (SKY and M-FISH) and CGH
Turid Knutsen

17.1 Introduction

17.2 Multicolor FISH (SKY/M-FISH)

17.3 Comparative Genomic Hybridization (CGH)




Contributed Protocols

18. Genomic Microarray Technologies for the Cytogenetics Laboratory
Bhavana J. Dave and Warren G. Sanger

18.1 Introduction

18.2 Applications

18.3 aCGH in a Cytogenetics Laboratory



Authors Note

Editors Note


19. Mathematics for the Cytogenetics Technologist
Patricia K. Dowling

19.1 General Concepts

19.2 Solutions

19.3 Statistical Tools

19.4 Using a Hemacytometer

19.5 Quantification and Purity Determination of DNA Using Spectroscopy


Additional Readings

20. Selected Topics on Safety, Equipment Maintenance, and Compliance for the Cytogenetics Laboratory
Helen Jenks and Janet Krueger

20.1 Introduction

20.2. Biological Hazard Safety

20.3 Chemical Safety

20.4 Fire Safety

20.5 Electrical Safety

20.6 Disaster Plan

20.7 Equipment Operation, Maintenance and Safety

20.8 Ergonomics

20.9 Regulatory Considerations



Contributed Protocols

21. A System Approach to Quality
Peggy J. Stupca and Sheryl A. Tran

21.1 Quality System

21.2. Process Management

21.3 Documents and Records

21.4 Assessments

21.5 Continual Improvement


Contributed Protocols

22. Laboratory Management
Mervat Ayad and Adam Sbeiti

22.1 Introduction

22.2 Management Concepts and Functions

22.3 Personnel Management

22.4 Quality Management and Control

22.5 Budget Development and Monitoring



Suggested Reading

23. Laboratory Information System
Peining Li and Richard Van Rheeden

23.1 Historical Perspective

23.2 General Description of LIS

23.3 LIS in Cytogenetics Laboratories

23.4 Trends for the Future LIS



24. Animal Cytogenetics
Marlys L. Houck, Teri L. Lear and Suellen J. Charter

24.1 Introduction

24.2 Domestic Animal Fertility

24.3 Captive Management

24.4 Wildlife Conservation

24.5 General Sample Collection Considerations

24.6 Fibroblast Cell Culture

24.7 Peripheral Blood Culture

24.8 Chromosome Analysis

24.9 Molecular and Comparative Cytogenetics




Contributed Protocols

25. Online Genetic Resources & References
Wahab A. Khan

25.1 Resource Information

Extra informatie: 
1218 pagina's
Januari 2017
666 gram
210 x 150 x 22 mm
John Wiley and Sons Ltd gb

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